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BD代理APC Mouse IgG2b κ Isotype Control   Clone  27-35货号：555745
规格：100罢
现货供应
中文名：APC标记Mouse IgG2b κ抗体

BD代理APC Mouse IgG2b κ Isotype Control   Clone  27-35货号：555745

Brand

BD Pharmingen™

Alternative Name

Anti-Dansyl

Vol. Per Test

20 µl

Isotype

Mouse C.SW IgG2b, κ

Application

Isotype control, Flow cytometry (Routinely Tested) 

Storage Buffer

Aqueous buffered solution containing BSA and ≤0.09% sodium azide.

Regulatory Status

RUO

BD代理APC Mouse IgG2b κ Isotype Control   Clone  27-35货号：555745

产物详情：

INTENDED USE

The mouse IgG2b, κ immunoglobulin isotype control monoclonal antibody 27-35 is specific for the hapten dansyl (5-[dimethylamino] naphthalene-1-sulfonyl). This hapten is not expressed on human cells or human cell lines. The 27-35 immunoglobulin was selected as an isotype control following testing which demonstrated low background staining on a variety of mouse and human tissues.

FORMAT

Format

APC

Excitation Source

Red 633 nm

Excitation Max

650 nm

Emission Max

660 nm

Allophycocyanin (APC), is an accessory photosynthetic pigment found in blue-green algae. Its molecular weight is approximay 105 kDa. APC has six phycocyanobilin chromophores per molecule, which makes it a very bright fluorochrome that is highly suitable for flow cytometry applications. Due to nearly identical excitation and emission properties but different spillover characteristics, APC and Alexa Fluor® 647 cannot be used simultaneously

Other Formats →

• APC-Cy™7
• APC-H7
• APC-R700
• Alexa Fluor® 488
• Alexa Fluor® 647

• Alexa Fluor® 700
• BB515
• BUV395
• BUV563
• BUV737

• BV421
• BV510
• BV650
• BV711
• BV786

• Biotin
• FITC
• PE
• PE-CF594
• PE-Cy™5

RESOURCES & TOOLS

• Download TDS

STORAGE AND HANDLING

The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated to APC under optimum conditions, and unconjugated antibody and free APC were removed. Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.

PRODUCT NOTICES

1. This reagent has been pre-diluted for use at the recommended Volume per Test. We typically use 1 × 10^6 cells in a 100-µl experimental sample (a test).
2. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
3. Please refer to www.bdbiosciences。。ｃｏｍ/pharmingen/protocols for technical protocols.
4. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences。。ｃｏｍ/colors.
5. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
6. Source of all serum proteins is from USDA inspected abattoirs located in the United States.